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    Welcome

    AMBP 2.0

    The Aquaculture Molecular Breeding Platform (AMBP) is a comprehensive portal for genetic data analysis in aquatic species of farming interest. It integrates pipelines for genotype imputation, kinship deduction, population structure inference, Genome-Wide Association Studies (GWAS), Genomic Selection (GS), and Genomic Mating (GM).
    We are pleased to announce the release of AMBP 2.0, which introduces new tools for image based phenotyping, genotype refinement, and breeding value prediction in a Smart Breeding Platform. In addition, we have updated the existing haplotype reference panels to incorporate structural variations and expanded the database to include 28 aquaculture species.

    Resources and Tools

    User Tutorial

    Phenotype Segmentation

    SV Imputation

    Genotype Refinement

    GEBV Prediction

    Reference Panel

    SV Search

    SNP Search

    SNP Imputation

    Genetic Structure

    Kinship

    GWAS

    Genomic Selection

    Genomic Mating

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    News and Updates

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    2021 MOE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China

    5 Yushan Road, Qingdao 266003, Shandong Province, China












    Smart Breeding Platform for Aquaculture

    The Smart Breeding Platform is an advanced tool integrated within the Aquaculture Molecular Breeding Platform (AMBP), designed to revolutionize genetic analysis in aquaculture. Leveraging cutting-edge artificial intelligence and deep learning technologies, this platform significantly enhances breeding programs by enabling high-precision phenotype segmentation, genotype refinement, and accurate breeding value predictions. The application of AI in breeding allows for the processing of vast datasets, improving the accuracy and efficiency of genomic selection and aiding in the identification of optimal breeding candidates. This platform not only offers robust tools for reference panel-based SNP imputation, but also incorporates the latest advancements in structural variation inference and reference-free genotype refinement, thus empowering users to make data-driven, informed decisions in genetic improvement strategies.

    Deep learning in phenotyping

    The Phenotype Segmentation pipeline utilizes advanced image-based segmentation techniques to automate the extraction and analysis of phenotypic traits in aquaculture species. By combining a binary level set method with the Mumford-Shah functional, this tool accurately segments fish bodies from background images, allowing precise measurements of key traits such as size, shape, and growth characteristics. Leveraging deep learning and mathematical optimization, the tool ensures high-throughput, reliable, and consistent phenotyping, significantly reducing manual labor and minimizing errors. This automated phenotyping process provides breeders with accurate, data-driven insights, enabling more informed decision-making in breeding programs, thereby enhancing genetic selection and improving breeding outcomes.
          The ocean harbors abundant biological resources, particularly aquatic species such as fish, shrimp, sea cucumbers, and scallops. However, the underwater environment is complex, and traditional image processing methods struggle to detect objects that are distorted or have colors similar to the surrounding environment. In this study, we captured images of fish in their natural underwater habitat and employed deep learning techniques to effectively delineate fish boundaries for body region segmentation. Using the area ratio, we developed a method to predict body weight, providing an efficient, non-invasive, and high-throughput approach to underwater feature measurement. Directly measuring weight in underwater environments is nearly impossible; however, the area ratio enables highly accurate biomass estimation, replacing manual detection and measurement. This significantly enhances the efficiency of biomass assessment.

    Original images
    Segmentation Result

    Genotype refinement

    The Genotype Refinement pipeline utilizes a denoising autoencoder model to impute and correct missing genotype data. Unlike traditional HMM-based methods, this tool is designed to handle incomplete data and reconstruct the original information from masked or corrupted inputs. It eliminates the need for a reference panel and captures complex, non-linear relationships in genomic data, particularly in regions with intricate linkage disequilibrium. By leveraging this advanced deep learning model, the tool significantly enhances the accuracy and efficiency of genotype imputation, providing breeders with high-quality, complete datasets for more informed genetic analysis and optimized breeding decisions.

    Deep leaning in genomic prediction

    The GEBV Prediction tool in the Smart Breeding Platform leverages advanced deep learning (DL) models, including Deep Neural Networks (DNNs), Convolutional Neural Networks (CNNs), and Recurrent Neural Networks (RNNs), to predict Genomic Estimated Breeding Values (GEBVs) for complex traits in aquaculture species. These models excel in genomic prediction by capturing non-linear relationships and intricate patterns in genomic data, which traditional methods often overlook.The tool addresses common challenges such as overfitting, which can occur with whole-genome SNP data, by incorporating dimensionality reduction strategies and sparse model structures. Techniques like minmax concave penalty (MCP) and sparse neural networks (SNNs) are applied to optimize model performance, improving prediction accuracy for critical traits such as growth, body morphology, and other essential aquaculture characteristics.By utilizing these state-of-the-art DL models, the GEBV Prediction tool enhances the accuracy and efficiency of genomic selection, empowering breeders to make more informed and precise breeding decisions, ultimately improving the genetic improvement of aquaculture species.




    Tools


    SNP imputation and SV identification

    Online Imputation

    Genotype imputation leverages local linkage disequilibrium to infer missing genotypes of target samples. In AMBP, users can carry out online imputation for 18 aquaculture species by two imputation tools.

    SNP Search

    Users can search SNPs by genomic coordinates and check their predicted effects or functions.

    SV identification

    Users can upload custom datasets to scan and identify simple structural variation (SV) and complex genomic rearrangements (CGR) by using ‘Starfish’ algorithm.

    Population characterizing

    Genetic Structure

    Users can investigate the population structure of target samples with genotype datasets. Genetic clustering is performed by genetic ancestry estimation and principal component analysis.

    Kinship

    Users can infer pairwise relationships from estimated IBD segments and kinship coefficients.

    Genetic breeding

    GWAS

    Users can upload custom datasets and scan markers across the whole genomes to locate genetic variations associated with a particular trait.

    Genomic Selection

    Users can upload custom datasets for Genomic Estimated Breeding Values (GEBV) prediction. AMBP includes three GS models: Genomic Best Linear Unbiased Prediction (GBLUP), Bayesian Lasso, and Sparse Neural Networks (SNN).

    Genomic Mating and Simulation

    Genomic mating (GM) represents an approach to maximize genetic gain while constraining inbreeding within a targeted range. Users can compare the genetic improvements made by GS and GM across multiple simulated generations.

    Download

    Datasets

    You can download the reference panel and the genome assemblies of each species



    Help


    Help

    Tutorial

    Detailed tutorial for each module.

    Updates

    The latest news and updated development history.

    FAQs

    Frequently asked questions and answers.

    Contact

    Ways to contact us about problems,comments and suggestions.